Methods in Capturing the Structure of a Protein

• Cryogenic Electron Microscopy (Cryo-EM)
• Nuclear Magnetic Resonance (NMR) Spectroscopy - Magnetic Resonance Spectroscopy (MRS)
• X-ray Crystallography

Methods Comparisons

• the biggest advantage of Cryo-EM to that of X-ray and NMR is that Cryo works great with larger proteins and specifically protein complexes
• Cryo-EM also works great with large unstructured and flexible proteins which are more or less impossible through X-ray