Cryogenic Electron Microscopy (Cryo-EM)

• is a cryomicroscopy technique applied on samples cooled to cryogenic temperatures and embedded in an environment of vitreous water / amorphous ice
• has attracted wide attention to the approach as an alternative to X-ray Crystallography or NMR Spectroscopy for macromolecular structure determination without the need for crystallization

Cryo-EM - Limitations

• Below 1 Å (angstrom) is almost impossible to reach for cryo-EM
• Sub-1.5-Å, or even sub-2-Å, resolution performance will remain accessible for some time to ONLY well-behaved samples